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Cells for measuring the aryl hydrocarbon receptor transcriptional activation amount, method and assay kit

机译:用于测量芳烃受体转录激活量的细胞,方法和测定试剂盒

摘要

According to one embodiment, a cell for measuring a level of Ah receptor transcriptional activation is provided. The cell is derived from a neural cell. The cell contains a chromosome into which a reporter construct and an Ah receptor gene are introduced. The reporter construct has a sequence represented by SEQ ID NO: 1 and a reporter gene operably linked to the downstream of the nucleotide sequence. The sequence represented by SEQ ID NO: 1 has a recognizing sequence of an Ah receptor and a nucleotide sequence which is operably linked to the downstream of the recognizing sequence and required to initiate transcription.
机译:根据一个实施方案,提供了一种用于测量Ah受体转录激活水平的细胞。该细胞源自神经细胞。该细胞包含一条染色体,在该染色体中引入了一个报告基因构建体和一个Ah受体基因。报告构建体具有由SEQ ID NO:1表示的序列和可操作地连接至核苷酸序列下游的报告基因。 SEQ ID NO:1表示的序列具有Ah受体的识别序列和与该识别序列的下游可操作地连接并且需要启动转录的核苷酸序列。

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