Apparatus, systems and methods for performing gel electrophoresis using a monolithic electrophoresis unit that at least includes first and second buffer chambers containing buffer solution and a gel chamber containing a pre-cast gel, whereby all of these chambers are integrated into a pre-fabricated single unit that is ready for use. In performing gel electrophoresis, a top seal of this monolithic electrophoresis unit is removed, followed by removing a gel chamber seal from over the gel chamber, such that, buffer solution contacts the pre-cast gel within such gel chamber. Target samples are loaded into the gel chamber for contact with the pre-cast gel, a reusable lid is attached to the top surface of the monolithic electrophoresis unit, and an electrical connection is provided through the reusable lid into the monolithic electrophoresis unit for performing electrophoresis on the pre-cast gel.
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