GENETICALLY MODIFIED SACCHAROMYCES CEREVISIAE FOR OVERPRODUCTION OF THE ENZYME CHITINASE AND PROCESS THEREOF

摘要

This invention describes the production of a novel genetically modified (GM) yeast which overexpresses the enzyme chitinase. A PCR amplified 1.6 kb gene fragment harbouring the chitinase gene including the introns from the thermophilic fungus, Thermomyces lanuginosus has been cloned into an E. coli vector pXcmknl2 by TA cloning method. The recombinant plasmid is named pXCHI-PPLV2. The recombinant plasmids were used to transform E. coli. The gene obtained from a recombinant plasmid is sequenced and DNA and deduced amino acid sequences are presented here. The fragment was also cloned into an yeast centromere plasmid and the yeast recombinant plasmid is named pLCHI-PPLV3. The recombinant plasmid was used to transform the yeast S. cerevisiae. The corresponding GMO YT5 carried the plasmid vector harbouring the thermophilic fungal chitinase gene. The GM yeast, overexpressing the chitinase enzyme is now available for industrial applications. A process to produce the enzyme in large scale from GM yeast and its applications in various industries is also described in this patent.