Genetically modified Saccharomyces cerevisiae useful for decomposing and utilizing kitchen waste, and method for constructing genetically modified Saccharomyces cerevisiae

摘要

The present invention relates to the field of genetic engineering and fermentation engineering, and further to a genetically modified Saccharomyces cerevisiae useful for the decomposition and utilization of kitchen waste. Recombinant Saccharomyces cerevisiae uses an Saccharomyces cerevisiae multigene co-expression vector to simultaneously introduce an α-amylase gene, a glucoamylase gene, and an acidic protease gene into Saccharomyces cerevisiae for accurate expression and Built by achieving secretion. Genes of the above three enzymes are simultaneously introduced into Saccharomyces cerevisiae to achieve expression and secretion, thereby giving recombinant Saccharomyces cerevisiae the ability to secrete amylase and protease. In this way, starch and protein in kitchen waste can be effectively decomposed into available carbon and nitrogen source materials, and an efficient conversion from kitchen waste to ethanol can be achieved. [Selection] Figure 1