AN IMPROVED BIOPROCESS FOR THE PRODUCTION OF L-METHIONINE AMINOPEPTIDASE (L-MAP) FROM STREPTOMYCES GEDANENSIS

摘要

The present invention provides an improved bioprocess for the production of L-methionine amino peptidase. More particularly it relates to an improved bioprocess for the production of extracellular L-methionine amino peptidase. The process comprises of cultivating a strain of Streptomyces gedanensis (ATCC 4880) in an inert solid support (polyurethane foam(PUF)), moistened with a nutrient solution composed of assimilable sources of carbon and nitrogen along with surfactant, growth promoting substances and mineral salts and subsequent isolation of methionine specific aminopeptidase from the fermented matter. Response surface methodology of Box Behnken design was employed to uncover the optimum level of significant factors which resulted in a maximum MAP production of 57 IU/g PUF.