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METHODS TO INCREASE THE CAPACITY OF HIGH CONTENT CELL-BASED SCREENING ASSAYS
METHODS TO INCREASE THE CAPACITY OF HIGH CONTENT CELL-BASED SCREENING ASSAYS
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机译:增加基于高含量细胞的细胞筛查能力的方法
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摘要
The present invention involves the pooling of multiple high content cell-based screening assays, and carrying out a primary screen in a one or more channels of a fluorescence detection device, which drastically increases the number of simultaneous high content cell-based screening events that can be carried out. Subsequent deconvolution of primary screen 'hits' (ie: those wells or locations on an array of locations in which the one or more test compounds caused a change in the fluorescence signal(s) from the fluorescent reporter molecules in the cells) enables much more rapid generation of high content cell screening data than was previously possible, and at significantly reduced costs.
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