The present invention provides a new strain of Brevibacillus later osporus LAK 1210 (MTCC 5487) having an accession number MTCC 5487, as a dual producer of insecticidal proteins and inducible chitinolytic enzymes with a potential utility in agriculture and forest insect pest management, plant disease control and mosquito control programs. The multiple effects of this new insecticidal strain of Brevibacillus laterosporus LAK 1210, as a chitinase producer with the ability to degrade marine wastes finds many biotechnological applications in the areas of agriculture, forestry, medicine and environment. Furthermore, the object of the invention is also directed to a new chitinase with novel characteristics from a newer source of microorganism and its novel applications in biomass utilization and waste management. Methods and compositions of the invention utilize the vegetative cells, their derivatives (spores, cellular extracts, enzyme fractions) and the polypeptides to develop a natural pesticide for control of insects and phytopathogenic fungi. Another object of this invention is to expand the synergistic effect of chitinases on insecticidal activity for an improved pesticidal activity where a wider range of pests are impacted.
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