METHOD FOR THE PURIFICATION AND STABILISATION OF ENZYME GLUCONATE DEHYDROGENASE (GADH, EC 1.1.99.3), ENZYME GLUCONATE DEHYDROGENASE (GADH, EC 1.1.99.3) AND THE USE OF ENZYME GLUCONATE DEHYDROGENASE (GADH, EC 1.1.99.3)

摘要

The invention relates to a method for the purification and stabilisation of enzyme gluconate dehydrogenase (GADH, EC 1.1.99.3), optionally recombinant, from multiple microorganisms, for example: Pseudomonas aeruginosa, Pseudomonas fluorescens, Gluconobacter oxydans, Gluconobacter industrius, Serratia marcescens, Klebsiella pneumoniae and Escherichia coli, and to the use thereof as a biological recognition element in biosensors for detecting gluconic acid in samples of interest. The invention also relates to a biocatalytic biosensor with free electrochemical transduction of interferences owing to the high selectivity of the enzyme obtained using the aforementioned method and stabilised with optimised chemical agents.