RECOMBINANT ANTIBODY FOR DIAGNOSING PORCINE EPIDEMIC DIARRHEA VIRUS USING A VIRUS SYSTEM AND AN INSECT CELL, AND A METHOD FOR PREPARING THE SAME

摘要

PURPOSE: A recombinant antibody for diagnosing porcine epidemic diarrhea virus is provided to easily maintain or store an antibody producing strain.;CONSTITUTION: A method for preparing a recombinant antibody for diagnosing porcine epidemic diarrhea virus comprises: a step of preparing a recombinant baculovirus vector containing a DNA fragment of sequence number 1; a step of transfecting baculovirus full length DNA Bac-N-Blue and the recombinant baculovirus vector into an insect cell and preparing recombinant baculovirus; and a step of culturing the recombinant baculovirus in the insect cell. The vector is pBlueBac4.5His. The insect cell is sf-9 cell.;COPYRIGHT KIPO 2013