TRANSFER VECTOR TO EXPRESS THE SINGLE CHAIN VARIABLE FRAGMENT OF NEUTRALIZING ANTIBODY AGAINST PORCINE EPIDEMIC DIARRHEA VIRUS ON THE BACTERIAL OUTER MEMBRANE AND THE E.COLI TO INHIBITING THE PROPAGATION OF PORCINE EPIDEMIC DIARRHEA VIRUS

摘要

A vector and Escherichia coli suppressing proliferation of PED virus are provided to neutralize specifically the porcine epidemic diarrhea virus by being transformed to a surface expression vector and to reveal short chain variable segments of a porcine epidemic diarrhea virus neutralizing antibody at a film surface of a bacterial body. A surface expression vector reveals short chain variable segments of porcine epidemic diarrhea virus neutralizing antibody consisting of a sequence 1 at a film surface of a bacterial body. The surface expression vector includes a base sequence consisting of a sequence 1 and coding the short chain variable cleavage site specifically neutralized to porcine epidemic diarrhea virus(PEDV), T7 RNA polymerase consisting of a base sequence of 21bp~40bp of a sequence 4 and revealing the short chain variable split gene at a film surface of a bacterial body, a base sequence consisting of A sequence 2 coding A signal peptide of IgA proteinase gene of a gonococcus and an autotransporter beta domain gene consisting of a sequence 3 of the IgA proteinase of a base sequence coding six histidines and a gonococcus inserted into a bacterial membrane.