S-selective highly active mutant arylmalonic decarboxylase

摘要

A mutant aryl malonate decarboxylase of S-selective highly active, the present invention provides an enzyme that produces the S-form of the optical purity of ee 100% almost α- arylpropionic acid from aryl malonate It is the following invention which are intended to. The following mutations: (i) capable of producing the S-form a α- arylpropionic acid aryl malonic acid, in the amino acid sequence of aryl malonic acid decarboxylase wild type as shown in SEQ ID NO: 2, and (i) (ii mutation), mutation (iii), or having mutations (i), (ii) and (iii), mutant aryl malonate decarboxylase S-selective and highly active (i): To cysteine ​​substitution of glycine (i) of the 74 th, and the substitution of glycine to the cysteine ​​of the first 188th; Substitution of other amino acid tyrosine (ii) of the first 48th; Substitution of other amino acid methionine (iii) of the 159 th.