Screening methods of making and Fab using it as well as the polynucleotide constructs may present the Fab in a cell-free translation system

摘要

Screening a Fab against the antigen of interest by ribosome display, mRNA display perform CIS and / or display using the polynucleotide construct or the following (1) and (2). (1) ribosome binding sequence, Fab first strand coding sequence, linker peptide coding sequence, the second strand coding sequence Fab, and includes monocistronic in this order scaffolding coding sequence, with the Fab which itself is encoded at the 3 'end polynucleotide construct having the structure necessary to maintain the complex. (2) ribosome-binding sequence, the second strand coding sequence or Fab Fab first strand coding sequence, and in this order the scaffolding coding sequence includes a second chain expression cistron and Fab Fab first chain expression cistron, Fab first expression 'has a ribosome stalling sequence ends, the second chain expression cistron Fab its 3' the 3 cistron polynucleotide construct having the structure necessary to maintain the complex with Fab which itself encodes the end.