Novel preparation method of Mulberry leaf extract for anti-hypertensive, anti-diabetic, and anti-aging and the product of the same

摘要

PURPOSE: A production method of a mulberry leaf extract is provided to mass-produce refined powder containing flavonoid with the high value from mulberry leaves. CONSTITUTION: Mulberry leaves harvested in June are pre-dried in the shade for a week, and dried in a 45-55C hot air drier for two days until the water content is 10 3%. 100 kg of dried mulberry leaves is mixed with 650 kg of 30% ethanol solution for ultrasound extracting for 12 hours at 40-50C, and the mixture is filtered and decompress-concentrated. The concentrate is dissolved in a 30% ethanol solution before settling for one night at room temperature, and centrifugal-separating precipitates. Supernatant is decompress-concentrated, and dissolved in a 20% ethanol solution. The concentrate passes through a Diaion HP-20 adsorption ion resin for absorbing flavonoid, and is eluted by 30% and 50% ethanol solutions. The silica gel and Sephadex column chromatography is conducted on each fraction successively. [Reference numerals] (AA) Mulberry leaves; (BB) Extraction (650 kg, 20% ethanol solution, ultrasound extractor, 50C, 12 hours, 2 times); (CC) Filtration; (DD) Filtrate; (EE,JJ) Decompress-concentration; (FF) Mulberry leaf ethanol extract (23 kg); (GG) Re-dissolving (100 kg, 30% ethanol solution); (HH) Settling for one night; (II) Supernatant; (KK) Mulberry leaf ethanol extract (21.2 kg); (LL) Suspension (50 kg, 20% ethanol solution); (MM) Diaion HP-20 column chromatography; (NN) 30% ethanol solution fraction; (OO) 50% ethanol solution fraction; (PP) Mulberry extract I (1.1 kg); (QQ) Mulberry extract II (0.73 kg)