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Norovirus detection method, and the preparing method of recombinant vector and recombinant protein for the detection

机译:诺如病毒的检测方法以及用于检测的重组载体和重组蛋白的制备方法

摘要

The present invention using a recombinant vector, and the vector for the detection of norovirus causing microorganisms known as sikjungdokgyun Production method of recombinant protein, and, to a detecting method of using the same Norovirus. Norovirus is a recombinant vector for production of recombinant proteins for detection of the present invention, Norovirus outer membrane protein (Norovirus Norwalk capsid protein) coding for either P1 or P2 domain of the domain containing the VP1 gene and the gene encoding the target protein and is characterized in that the production so that the gene is expressed in a fused form. In addition, recombinant protein production method for detecting norovirus of the present invention, the E. coli transformed with the recombinant vector, and from which the norovirus molecular diagnostic characterized in that for producing a recombinant protein. ;
机译:本发明使用重组载体,以及用于检测引起诺如病毒的微生物的载体,被称为sikjungdokgyun。重组蛋白的生产方法,以及使用该诺如病毒的检测方法。诺如病毒是用于产生用于检测本发明的重组蛋白的重组载体,诺如病毒外膜蛋白(Norovirus Norwalk衣壳蛋白)编码包含VP1基因和编码靶蛋白的基因的结构域的P1或P2结构域,并且其特征在于产生使基因以融合形式表达。另外,本发明的用于检测诺如病毒的重组蛋白的生产方法是用重组载体转化大肠杆菌,并且根据诺如病毒的分子诊断,其特征在于用于生产重组蛋白。 ;

著录项

  • 公开/公告号KR101441950B1

    专利类型

  • 公开/公告日2014-09-25

    原文格式PDF

  • 申请/专利权人

    申请/专利号KR20120113616

  • 发明设计人 류명이;박종필;

    申请日2012-10-12

  • 分类号C12N15/63;C12N15/62;C07K19/00;G01N33/569;

  • 国家 KR

  • 入库时间 2022-08-21 15:40:08

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