A method for diagnosing pathology of the neuromuscular apparatus in neurodegenerative diseases in a genetic model of Alzheimer's disease and amyotrophic lateral sclerosis ON ANIMALS

摘要

A method for diagnosing pathology of the neuromuscular system in neurodegenerative diseases in genetic models of Alzheimer's disease (AD) and amyotrophic lateral sclerosis (ALS) transgenic mice including the study of peripheral tissue, characterized in that produce study tissue neuromuscular system, namely: the-first, exploring quantum release of the neurotransmitter acetylcholine at the neuromuscular synapse: mice models of ALS and BA defined emission reduction neurotransmitter twice; Second, estimate the number of neuromuscular synapses which occurs in the loading of the fluorescent dye FM 1-43, as well as the degree of loading of FM 1-43: mice models of AD and ALS occurs significant reduction in the number of synapses with FM 1-43 and loading / or reduced degree of degree of loading of FM 1-43; Third, the metabolism of cholesterol tested membrane at the neuromuscular synapse: mice models of AD and ALS violation observed distribution and reduction of membrane cholesterol by 25-35% compared to wild-type mice, as measured resting membrane potential of cells of skeletal muscle fibers : mice models of AD and BAS observed decrease in membrane potential of not less than 10 mV, as a result of pathology detection achieved neuromuscular system in neurodegenerative diseases and in models of asthma have ALS trans ennyh mice.