METHOD OF PCR-RFLP genotyping CATTLE ON A allele K DGAT1 gene

摘要

A method for conducting PCR-RFLP Genotyping cattle alleles A and K DGAT1 gene, characterized in that the other sequences of oligonucleotide primers used in the PCR step: DGAT1-1: 5'-CCGCTTGCTCGTAGCTTTCGAAGGTAACGC-3 '(SEQ ID NO: 1) DGAT1 -2: 5'-CCGCTTGCTCGTAGCTTTGGCAGGTAACAA-3 '(SEQ ID NO: 2) DGAT1-3: 5'-AGGATCCTCACCGCGGTAGGTCAGG-3' (SEQ ID NO: 3), and in step RFLP applies another restriction endonuclease - TaqI, with generation genotype -specific fragments genotype AA = 82/18 bp, genotype CC = 100 bp, and genotype AA = 100/82/18 bp.