[Objective] The paper was to study the genetic effects of ANGPTL4 gene in Guizhou yellow cattle. [Method] The polymorphisms of ANGPTL4 gene in 46 individuals of Guanling yellow cattle and Sinan yellow cattle were detected using PCR-RFLP assay. [Result] A fragment of 260 bp was amplified from ANGPTL4 gene. After digestion by restriction endonuclease Mlu CI,two genotypes,AA and AB,were produced whereas BB genotype was not found. A mutation of 205T→C was observed through sequencing of different genotypes. AA genotype was dominant in both yellow cattle breeds,and A allele frequency was higher than B allele frequency. χ~2 test results showed that the two cattle breeds were at Hardy-Weinberg equilibrium state in the digestion site( P > 0. 05). [Conclusion]The effective molecular markers of Guizhou yellow cattle,such as growth trait and intramuscular fat content,are obtained,which will provide a theoretical basis for molecular breeding of meat performance of Guizhou yellow cattle.
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