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METHOD FOR INTRACELLULAR DNA AMPLIFICATION

机译:细胞内DNA扩增的方法

摘要

PROBLEM TO BE SOLVED: To provide a DNA amplification method useful to acquire, in a short period of time, microorganisms which highly produce target substances.SOLUTION: The invention provides a method for intracellular DNA amplification which amplifies a DNA to be amplified under existence of a DNA encoding a protein selected from proteins consisting of an amino acid sequence having a specific sequence, a protein comprising an amino acid sequence in which one or more amino acid of the amino acid sequence is deleted, substituted, inserted or added, or a protein comprising an amino acid sequence having 90% or more identity to the amino acid sequence, a DNA consisting of a specific base sequence, or a DNA subjected to hybridization in a condition of being stringent to the specific base sequence.
机译:解决的问题:提供一种DNA扩增方法,该方法可用于在短时间内获取高产目标物质的微生物。解决方案:本发明提供一种细胞内DNA扩增的方法,其在存在DNA的情况下扩增待扩增的DNA。编码蛋白质的DNA,该蛋白质选自具有特定序列的氨基酸序列的蛋白质,包含其中氨基酸序列的一个或多个氨基酸被缺失,取代,插入或添加的氨基酸序列的蛋白质,或蛋白质包含与该氨基酸序列具有90%或更多的同一性的氨基酸序列,由特定碱基序列组成的DNA或在对该特定碱基序列严格的条件下进行杂交的DNA。

著录项

  • 公开/公告号JP2015070845A

    专利类型

  • 公开/公告日2015-04-16

    原文格式PDF

  • 申请/专利权人 MEIJI SEIKA PHARMA CO LTD;

    申请/专利号JP20140228366

  • 发明设计人 YANAI KOJI;MURAKAMI TAKESHI;SUMIDA NAOMI;

    申请日2014-11-10

  • 分类号C12N15/09;C12N1/21;C12N1/15;C12N1/19;C12N5/10;C07K14/36;

  • 国家 JP

  • 入库时间 2022-08-21 15:34:47

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