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The DNA amplification method of the intracellular

机译:细胞内DNA的扩增方法

摘要

The present invention relates to a method to efficiently amplify the DNA of large size. In particular, the present invention is a DNA amplification method in a cell, in the presence of DNA of the following (i), (ii) and is selected from (iii), be amplified the DNA to be amplified It is characterized by, relates to a DNA amplification method: DNA encoding a protein selected from the 3) 1 (i) below), and 2): Protein comprising the amino acid sequence set forth in 1) SEQ ID NO: 1, Protein comprising an amino acid sequence by deletion, substitution, or insertion of one or more amino acids of the amino acid sequence set forth in 2) SEQ ID NO: 1, Protein comprising an amino acid sequence having 90% or more to the amino acid sequence set forth in 3) SEQ ID NO: 1, DNA consisting of a nucleotide sequence described in (ii) SEQ ID NO: 2, DNA that hybridizes under stringent conditions to the nucleotide sequence set forth in (iii) SEQ ID NO: 2.
机译:本发明涉及有效扩增大尺寸DNA的方法。特别地,本发明是在以下(i),(ii)的DNA的存在下且选自(iii)的DNA的细胞中的DNA扩增方法,其被扩增的DNA的特征在于,涉及一种DNA扩增方法:编码选自以下3)1(i)和2)的蛋白质的DNA:包含1)SEQ ID NO:1所示氨基酸序列的蛋白质通过缺失,取代或插入2)SEQ ID NO:1中所示氨基酸序列的一个或多个氨基酸,蛋白质包含相对于3)中所述氨基酸序列具有90%或更多氨基酸的氨基酸序列SEQ ID NO:1,由(ii)SEQ ID NO:2中所述的核苷酸序列组成的DNA,在严格条件下与(iii)SEQ ID NO:2中所示的核苷酸序列杂交的DNA。

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