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use of corn untranslated region for transgene expression in plants

机译:玉米非翻译区在植物中转基因表达的应用

摘要

for the expression of recombinant nucleic acid sequences are enhanced in genetically modified plants and genetically modified plant tissue method, vector and gene construct is provided. According to the present invention, various nucleic acid sequences are obtained from the 3 'untranslated region of the gene encoding a ubiquitin-protein and / or retrieved, so as to be adjacent to a respective portion of the selected coding region of the vector It is operated. Vector construct may be introduced into a plant and / or plant tissues by conventional procedures or gene targeting procedures by which leads to enhanced expression of a selected coding region. In some embodiments, the coding region is selected, the expression of one or more proteins are known to impart insecticidal activity of a given level in the transgenic plants and / or transgenic plant tissue It is a chimeric gene or gene fragment.
机译:用于在转基因植物中增强重组核酸序列表达的方法以及转基因植物组织的方法,载体和基因构建体。根据本发明,从编码遍在蛋白的基因的3'非翻译区获得各种核酸序列和/或将其回收,以使其与载体的所选编码区的各个部分相邻。经营。可以通过常规方法或基因靶向方法将载体构建体引入植物和/或植物组织中,从而导致所选编码区的表达增强。在一些实施方案中,选择编码区,已知一种或多种蛋白质的表达在转基因植物和/或转基因植物组织中赋予给定水平的杀虫活性。它是嵌合基因或基因片段。

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