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Retro Diels-Alder reaction of a cleavable linker in DNA / RNA applications
Retro Diels-Alder reaction of a cleavable linker in DNA / RNA applications
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机译:DNA / RNA应用中可裂解连接子的逆Diels-Alder反应
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摘要
The present invention provides a novel approach to reversibly conjugated oligonucleotide, the method comprising: obtaining a target object to be labeled with the labeled oligonucleotide and the dienophile moiety with the diene moiety, the oligonucleotide and the labeled with the diene moiety A step of heating at a first temperature the labeled target subject in solution in the dienophile moiety, comprising the steps of causing a Diels-Alder reaction to produce the conjugate, in a step of heating the above conjugated to a second temperature The matching includes a step of generating a retro Diels-Alder reaction to reproduce a target subject labeled oligonucleotide and the dienophile moiety labeled with the diene moiety, to provide a novel approach. .BACKGROUND
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