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RETRO DIELS ALDER REACTION AS A CLEAVABLE LINKER IN DNA/RNA APPLICATIONS
RETRO DIELS ALDER REACTION AS A CLEAVABLE LINKER IN DNA/RNA APPLICATIONS
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机译:还原狄尔斯反应作为DNA / RNA应用中的可裂解连接物
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摘要
The invention provides a novel approach for reversibly conjugating an oligonucleotide, which includes obtaining an oligonucleotide labeled with a diene moiety and a target entity labeled with a dienophile moiety; heating the oligonucleotide labeled with the diene moiety and the target entity labeled with the dienophile moiety in a solution at a first temperature to effect Diels Alder reaction to produce a conjugate; and heating the conjugate to a second temperature to effect retro Diels Alder reaction to regenerate the oligonucleotide labeled with the diene moiety and the target entity labeled with the dienophile moiety.
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