RETRO DIELS ALDER REACTION AS A CLEAVABLE LINKER IN DNA/RNA APPLICATIONS

摘要

The present invention strikes and oligonucleotides labeled with a diene moiety as two yen body residues to yield the labeled target material ; Preparing a conjugate by performing Diels Alder reaction the diene -labeled oligonucleotide , and strikes the labeled target moieties to heat the body in two yen sieve residue in a solution of a first temperature ; And heating the assembly to a second temperature and by performing the retro Diels Alder reaction diene including the step of playing the labeled oligonucleotide and strikes the labeled target moieties to the body member in a two yen residues , up to the junction nucleotide reversibly the novel approach to be about .