Provided herein is a method for detecting porphyrin using LC-MS/MS by pretreating a biological sample. This method is very effective in detecting a small amount of porphyrin. Accordingly, it is possible to detect porphyrin from biological samples which contain a small amount of porphyrin such as blood. Furthermore, even in the case where the number of times obtaining biological samples and the amount of obtainable biological samples are very limited, such as newborn infants, a precise detection of porphyrin is possible. Hence, this method can be utilized for early diagnosis of porphyrin-related diseases.
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