The present invention relates to bio-hydrogen by an electrochemically active biofilm, an electron donator, and (+)-charged metal nanoparticles and to a method for producing the same. The bio-hydrogen according to the present invention is produced by using an electrochemically active biofilm, an electron donator, and (+)-charged metal nanoparticles, so that no any harmful chemicals and external energy input are required. The bio-hydrogen is very effective because a total hydrogen yield reaches 44%.
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