Provided in the present invention are a composition for detecting chiral amino acid and a method for detecting chirality of amino acid using the same. The composition for detecting chiral amino acid includes metal nanoparticles having the surface modified by cis-mercapto-L-proline represented by chemical formula 1, and detects chirality of amino acid by using colorimetric signal mirror image excessive amount of amino acid. (Here, X is HS.);COPYRIGHT KIPO 2015
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