STRAIN Francisella tularensis 15 / 23-1 ∆recA WITH REDUCED REACTOGENICITY TO CREATE A LIVING TULAREMIA VACCINE AND METHOD FOR ITS PRODUCTION

摘要

1. The strain Francisella tularensis 15 / 23-1ΔrecA with reduced reactogenicity to create a live tularemia vaccine, deposited in the State collection "GKPM-Obolensk", registration number B-6623.2. A method of obtaining a Francisella tularensis 15 / 23-1ΔrecA strain with reduced reactogenicity to create a live tularemia vaccine from the vaccine strain F. tularensis 15 NIIEG consists in sequential allelic exchange of one of two copies of the iglC gene and then the recA gene for their deleted variants using a suicide vector plasmid characterized in that the vector pGM5 constructed on the basis of the bireplicon plasmid pHV33 with the sacB gene introduced into it and deletion in the ori region of pC194, followed by the introduction of tularemia, is used as a suicide plasmid microbial transformation method.