VECTOR FOR ACETATE KINASE GENE KNOCKOUT IN Clostridium thermocellum, HOST CELL, GENETICALLY MODIFIED MICROORGANISM Clostridium thermocellum, METHOD OF PRODUCING SAID MICROORGANISM AND METHOD OF CONVERTING LIGNOCELLULOSE BIOMASS TO ETHANOL

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to biotechnology and a vector, a host cell containing a vector, a genetically modified microorganism Clostridium thermocellum, a method of producing said microorganism and a method of converting lignocellulose biomass to ethanol. The present vector is designed for acetate kinase gene knockout in Clostridium thermocellum and has nucleotide sequence SEQ ID NO:1 or SEQ ID NO:2. The present genetically modified microorganism is transformed by the nucleotide sequence SEQ ID NO:1 or SEQ ID NO:2, and can also further contain a non-native gene which provides the capacity to metabolise pentose sugar, hydrolyse xylan or is involved in metabolic production of ethanol. The present method of converting lignocellulose biomass to ethanol includes bringing the biomass into contact with said genetically modified microorganism.;EFFECT: invention enables to obtain a larger amount of ethanol.;17 cl, 53 dwg, 6 tbl, 10 ex