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METHOD FOR DETERMINING LENGTH POLYMORPHISM OF CR1 RECEPTOR.

机译:确定CR1受体长度多态性的方法。

摘要

The invention relates to a method for determining the length polymorphism of the CR1 receptor, which comprises three domains, LHR A, LHR C and LHR D, and n LHR domain B, where n is an integer between 0 and 3. From genetic material, a single pair of specific primers amplifies a first segment of the LHR B domain (variant segment B) and a second segment belonging to at least one other domain (non-variant segment), said variant segment B and said non-variant segment having, outside the hybridization zones of said primers, at least three nucleotide differences. Using a quantitative tool, the number of variant B segment and non-variant segment amplicons is deduced, the variant / non-variant ratio being different depending on the length polymorphism of the CR1 receptor.
机译:本发明涉及一种确定CR1受体的长度多态性的方法,其包括三个结构域:LHR A,LHR C和LHR D,以及n个LHR结构域B,其中n是0至3之间的整数。单对特异性引物扩增LHR B结构域的第一区段(变异区段B)和属于至少一个其他结构域的第二区段(非变异区段),所述变异区段B和所述非变异区段具有:在所述引物的杂交区之外,至少三个核苷酸差异。使用定量工具,推导变体B区段和非变体区段扩增子的数量,其变体/非变体比率根据CR1受体的长度多态性而不同。

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