OPTICAL MICROSCOPE DEVICE, MICROSCOPY METHOD, AND COMPUTER PROGRAM FOR MICROSCOPY USING SINGLE-LIGHT-EMITTING-PARTICLE DETECTION TECHNOLOGY

摘要

There is provided a microscopic observation technique capable of detecting a light-emitting object or a light-emitting particle whose position varies dynamically in a thick sample by using the way of the scanning molecule counting method. In the optical microscopic observation technique according to the present invention, a light-emitting particle is detected by detecting the light from the light-emitting particle moving in a liquid with the optical system of a confocal microscope or a multiphoton microscope. In processing operations, the light from the light detection region is detected while moving the position of the light detection region in each observed subregion obtained by dividing a region to be observed of the microscope into plural regions; the signal of the light from a light-emitting particle is individually detected; and the position of the light-emitting particle corresponding to the detected signal is determined in the region to be observed. The moving of the position of the light detection region in each observed subregion is performed continuously in at least two directions or and/or continuously multiple times in each observed subregion.