Recombinant microorganism for the preparation of terpenoids, and method for constructing a recombinant microorganism

摘要

Recombinant strains for the preparation of a terpenoids, and how to construct the recombinant strain is provided. Further, the recombinant bacterium 1 is provided a recombinant bacterium obtained in order to improve the enzymatic activity of - ketoglutarate dehydrogenase in E. coli or a variant thereof. How to improve the enzymatic activity of - ketoglutarate dehydrogenase in E. coli or a variant thereof, the original of the regulatory elements of ketoglutarate dehydrogenase gene in E. coli or a variant thereof (sucAB), artificial regulatory elements M1-46, artificial regulatory elements M1-37, and it is to be replaced with any of the artificial regulatory elements M1-93. Further, a plurality of recombinant bacteria is provided. - ketoglutarate dehydrogenase, succinate dehydrogenase, and to improve the activity of transaldolase, by improving the ability to synthesize NADPH and ATP of the cells, the production capacity of the efficiency and terpenoids of the MEP pathway is improved.BACKGROUND