Concentrated and next-generation sequencing all nucleic acids, including both genomic DNA and CDAN

摘要

present invention, a mixture of nucleic acids comprising the genomic DNA sequence and cDNA sequence obtained from the test sample is concentrated, to a method of performing sequencing. In the present application, an aspect, from the test sample (e.g., the test human samples, etc.), a method of obtaining a population of nucleic acid of interest has been concentrated, the genomic DNA sequence and cDNA sequence obtained from (a) the test sample sufficient (b) a mixture of nucleic acids, a set of probes that are complementary to the nucleic acid of interest present in the mixture of the nucleic acid, to the nucleic acid and said probe hybridizes; mixture the step of providing a nucleic acid comprising step contacted in such conditions; separated and (c) the probe and the hybridized nucleic acid from the unhybridized nucleic acid, thereby providing a method comprising obtaining a population of nucleic acid of interest has been concentrated .