device for optically controlling nucleic acid sequencing, in particular for optically controlling an iterative step reaction for determining a sequence of a nucleic acid by synthesis, method for optically controlling nucleic acid sequencing, in particular for controlling of an iterative step reaction to determine a sequence of a nucleic acid by synthesis, a program element for the optical control of a computer readable DNA sequencing, in which a computer program for the optical control of a DNA sequencing dna and use of a portion as a blocking portion in DNA sequencing

摘要

device for optically controlling nucleic acid sequencing, in particular for optically controlling an iterative step reaction to determine a sequence of a nucleic acid by synthesis, method for optically controlling nucleic acid sequencing, in particular for optical control of an iterative step reaction to determine a sequence of a nucleic acid by synthesis, a program element for the optical control of a computer readable DNA sequencing, in which a computer program for the optical control of a sequencing DNA use and a portion as a blocking moiety in DNA sequencing The present invention relates to DNA sequencing with reagent cycling in the yarn grid. the suggested sequencing approach whereby a single fluid with no washing step is allowed to be used. Based on the strong optical confinement and the excitation light and cleavage light, the sequencing reaction can be read without surface washing. step sequencing is achieved by the use of nucleotides with optically cleavable blocking moieties. after reading, the incorporated into the nucleotide is unlocked by cleavage light through the same substrate. This ensures that only linked nucleotides are unlocked.