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Culture quality evaluation method

机译:文化素质评价方法

摘要

A culture quality evaluation method comprises: a first step of culturing a pluripotent stem cell under a predetermined culture condition and creating a sample; a second step of imaging a proliferated cell colony in the sample and accordingly capturing an original image; a third step of dividing the original image into smaller images of a predetermined size and calculating standard deviations of pixel values of pixels of the smaller images; and a fourth step of judging whether the sample is an acceptable sample based upon a ratio of a number of the smaller images whose values of the standard deviations are within a predetermined range to a total number of the smaller images.
机译:一种培养质量评估方法,包括:在预定培养条件下培养多能干细胞并制备样品的第一步;第二步是对样品中增殖的细胞集落进行成像,并相应地捕获原始图像;第三步,将原始图像划分为预定大小的较小图像,并计算较小图像的像素的像素值的标准差;第四步骤,基于所述标准差值在预定范围内的较小图像的数量与所述较小图像的总数的比值,判断所述样品是否为可接受的样品。

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