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Immunological detection method and detection kit for Mycobacterium tuberculosis group

机译:结核分枝杆菌群的免疫学检测方法及检测试剂盒

摘要

The conventional detection method for Mycobacterium tuberculosis group takes a long time and requires a great deal of labor and cost, so that a definite diagnosis of tuberculosis and early treatment start cannot be performed, and a rapid detection method is provided. Was desired. In direct detection from a biological sample, if MPT64 is not present or very small in the biological sample, it cannot be detected. There was a need for a new detection method. [MEANS FOR SOLVING PROBLEMS] By subjecting a biological sample containing Mycobacterium tuberculosis group to heat treatment, the Mycobacterium tuberculosis-specific group secreted protein, particularly MPB64, is secreted outside the bacterial body, and the obtained processed sample is subjected to immunological measurement. Provides a method and a kit for detecting the Mycobacterium tuberculosis more quickly and easily without culturing a biological sample. [Selection figure] None
机译:用于结核分枝杆菌群的常规检测方法花费时间长并且需要大量的劳力和成本,从而不能进行明确的结核病诊断和早期治疗开始,并且提供了一种快速的检测方法。想要的。从生物样品中直接检测时,如果在生物样品中不存在MPT64或微量MPT64,则无法检测到。需要一种新的检测方法。 [解决问题的方法]通过对含有结核分枝杆菌基团的生物样品进行热处理,结核分枝杆菌特异性基团分泌的蛋白质,特别是MPB64,被分泌到细菌体外,并对获得的加工样品进行免疫学测定。提供了一种无需培养生物样品即可更快速,更轻松地检测结核分枝杆菌的方法和试剂盒。 [选择图]无

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