METHOD FOR THE MICROSCOPE IMAGING OF SAMPLES ADHERING TO BOTTOMS OF FLUID FILLED WELLS OF A MICROTITER PLATE

摘要

A method for microscope imaging of a sample, wherein a sample well is filled with liquid with the sample adhered to the bottom, illuminating and imaging the sample well from the underside and capturing at least one sample image thereof, wherein any inhomogeneity of the illumination is equalized by providing a test well with the same structure filled with liquid but no sample, making a reference measurement of the test well by illuminating the test well, imaging the illuminated test well from the underside and capturing a reference image which covers the entire bottom, analyzing the reference image to determine a brightness correction specification based on brightness fluctuation, and using the brightness correction specification to correct the sample image, including determining a position of at least a part of the sample image and using a value of the brightness correction specification assigned to the position.