Process for the microscopic imaging of samples on the bottoms of fluid-filled pots of a microtiter plate

摘要

Method for microscopic imaging of a sample (14) which adheres to the bottom (3) of the potty (1) in a potty (1), the following steps being carried out for microscopic imaging of the sample (14): (a) the potty (1 ) is illuminated with illuminating radiation (2) and (b) the illuminated base (3) of the potty (1) is magnified from the underside (15) and an image (23a) of the illuminated base (3) is recorded, characterized in that that a potty-caused nonuniformity in the illumination of the floor (3) is compensated for by (c) providing a sample-free test potty (22) which, apart from the missing sample, corresponds to the potty to be microscopic (1), (d) on the test Potty (22), a reference measurement is carried out by means of steps (a) and (b), a reference image (24) is recorded, which shows the entire floor (3), (e) in the reference image (24) a brightness correction information is determined , the Helligke Its correction indication indicates a brightness fluctuation as a function of the location on the bottom (3) of the test potty (22), (f) the image (23b) of the bottom (3) of the sample-containing potty (1) is corrected by means of the brightness correction indication, the position of the image (23b) on the ground (3) and the value of the brightness correction information belonging to this position is used.