Methods for the production of hormones and / or purification

摘要

1. This instruction relates to the method of producing and / or purifying hormone, especially stimulating hormone (FSH);The use of human cell platforms and the resulting products and their use, produced in humans or animals. 13. Claim 3: hormone production method, comprising the following steps: (1) complete extraction of RNA from tissue samples; (2) step 1 reverse transcription of adnc synthesis from RNA; (3) adnc fragment amplification of complete chains (A and b); (4) cloning In each amplified fragment (A and b) of mammalian cell expression vector; (5) joint transfer of two vectors containing chain A and chain B,Together with vectors containing antibiotic resistance genes in human embryonic kidney cells (HEK) or derived cells; (6) cloning of recombinant cells by antibiotic resistance selection; (7) selective detection of recombinant protein expression by Western blotting; and 8. Selective purification of hormones. 11. Claim 10: hormone purification method, including dividing the excess culture containing recombinant hormone into the following stages: (a) dye affinity chromatography;(b) Ion exchange chromatography (c) size exclusion chromatography - claim 22: method for direct analysis of hormones in a cell culture environment, including liquid chromatography with UV detection (254 nm) and column c83216a;where the runs are carried out with a mobile phase of methanol, ethanol and water, flow rate of 1 ml / min, oven temperature 40 ° C and sample injection volume of 20 ml.