A process for obtaining hmg-up and non pollutant components

摘要

This application relates to a purification method of (Human menopausal gonadotropin (HMG) for multimodal Chromatography to obtain a Composition of HMG - up (Human menopausal gonadotropin with degree of ultrapureza).Claim 1: a process for obtaining composition (Human menopausal gonadotropin (HMG - up with degree of ultrapureza) free of contaminants such as non gonadotropin plasma serine protease inhibitor, afamina, Binding Protein with insulin-like growth factor 7, - 2 glycoprotein of zinc and AlbuminWhere a Composition of gonadotropin is subjected to the following steps: (i) purification of pollutants gonadotropin using a strong anion Exchanger with multimodal functionality.Eluting buffer with a pH at a constant but changing the conductivity to obtain a fraction J3 - up only LH bioactivity; (ii) obtaining a fraction of HMG - up on stage by a Balanced mixture of bioactivities equivalent FSH from the fraction J2 With LH of the J3 fraction up obtained; (iii) subjecting The fraction of HMG - up Stages: 1) sterile Filtration, Nanofiltration, 2) and 3) precipitation; to obtain a Composition of HMG - up (Human menopausal gonadotropin with degree of ultrapureza) free of contaminants such as non gonadotropin protease inhibitor, afamina, Binding Protein with growth factor Insulin, type 7, - 2 - glycoprotein of zinc and Albumin.