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Automated analysis of patterns of probe-target interaction multiplexed: pattern matching and identification of alleles
Automated analysis of patterns of probe-target interaction multiplexed: pattern matching and identification of alleles
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机译:探针-靶标相互作用的模式自动化分析:模式匹配和等位基因鉴定
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摘要
Method for assignment of alleles where assignments are made based on subjecting a sample comprising target nucleic acid hybridization assay or elongation assay mediated capture, or both, and where several polymorphic present markers are analyzed alleles nucleic acid target, the method comprising: determining an intensity pattern of test signals generated by interactions probe-target to generate a reaction pattern designating as positive those signal strengths exceeding a predetermined threshold and designating as negative those signal strengths do not exceed the predetermined threshold binarizing thereby said intensity pattern test signals generated interactions probe-target wherein each of said intensity corresponds to the number of hybridization events corresponding elongation or, as applicable; make assignments of alleles based on compare and match said pattern of reaction with a reaction pattern similar reference representing combinations of two or more reference alleles as listed in a reference collection of alleles for the target nucleic acid building; and designate, as new alleles, which have reaction patterns differing patterns reference reaction representing combinations of two or more alleles reference by a predetermined minimum disparity.
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