首页> 外国专利> METHOD FOR DETECTING NUCLEIC ACID, CAPTURE PROBE, DETECTION PROBE SET, MICROARRAY, NUCLEIC ACID DETECTION KIT, NUCLEIC-ACID-IMMOBILIZED SOLID PHASE, AND FLUID DEVICE

METHOD FOR DETECTING NUCLEIC ACID, CAPTURE PROBE, DETECTION PROBE SET, MICROARRAY, NUCLEIC ACID DETECTION KIT, NUCLEIC-ACID-IMMOBILIZED SOLID PHASE, AND FLUID DEVICE

机译:检测核酸,捕获探针,检测探针组,微阵列,核酸检测试剂盒,核酸固定化固相和流体装置的方法

摘要

A method for detecting a nucleic acid, having the following steps: a) a step for bringing a solution including a nucleic acid and a detection probe labeled with a labeling substance into contact with a solid phase on which a capture probe is immobilized, the capture probe having a first portion comprising a sequence capable of hybridizing with the nucleic acid, a second portion comprising a sequence capable of hybridizing with the detection probe, and a stem part for forming a double strand; b) a step for hybridizing the nucleic acid with the first portion, hybridizing the detection probe with the second portion, and forming a nucleic acid-detection probe-capture probe complex on the solid phase; c) a step for ligating the nucleic acid and the detection probe; d) a step for eliminating bonding between the capture probe and the detection probe not ligated with the nucleic acid; and e) a step for detecting the labeling substance in the complex.
机译:一种用于检测核酸的方法,其具有以下步骤:a)使包含核酸和被标记物质标记的检测探针的溶液与固定有捕获探针的固相接触的步骤。探针,其第一部分包含能够与核酸杂交的序列,第二部分包含能够与检测探针杂交的序列,以及用于形成双链的茎部分; b)使核酸与第一部分杂交,使检测探针与第二部分杂交,并在固相上形成核酸-检测探针-捕获探针复合物的步骤; c)连接核酸和检测探针的步骤; d)消除捕获探针和未与核酸连接的检测探针之间的结合的步骤; e)检测复合物中标记物质的步骤。

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