BLOOD-VESSEL THERAPEUTIC PATCH COMPOSED OF ENDOTHELIAL CELLS DERIVED FROM IPSCS PRE-TREATED WITH LEPTIN

摘要

Described herein are compositions and techniques related to generation of endothelial cells from pluripotent stem cells. Via leptin addition and activation of the canonical JAK/STAT pathway, vascular density improves in leptin-treated EBs compared to untreated controls in murine and human cells. Endothelial cell and angiogenesis marker expression analyzed via qRTPCR demonstrated elevated CD31 and phosphoSTAT3 (pSTAT3) expression. Such results allow for generation of therapeutic microvessels derived from pluripotent stem cells with enhanced capacity for angiogenesis and vasculogenesis, in addition to in vivo capillary network formation, thereby finding regenerative medicine applications for diseases such as diabetes and conditions such as burn victims.