HOMING OF AUTOLOGOUS CELLS TO TARGET ZONE IN TISSUE USING ACTIVE THERAPEUTICS OR SUBSTANCES BY ISOLATING ENDOTHELIAL PROGENITOR CELLS OR BONE MARROW DERIVED STEM CELLS FROM MAMMAL DELIVERING CYTOKINE OR CHEMOATTRACTANT TO TARGET ZONE OF TISSUE AND THEN REINTRODUCING ISOLATED ENDOTHELIAL PROGENITOR CELLS OR BONE MARROW DERIVED STEMS CELLS TO MAMMAL

摘要

PURPOSE: A method for inducing vascular growth in tissue of a mammal by isolating endothelial progenitor cells or bone marrow derived stem cells from the mammal, delivering a cytokine or chemoattractant to a target zone of the tissue and then reintroducing the isolated endothelial progenitor cells or bone marrow derived stems cells to the mammal. It permits homing of the endothelial progenitor cells or bone marrow stem cells to the target zone of the tissue to induce vascular growth in tissue of the mammal and is effective in minimally invasive treatment of cardiac ischemia. CONSTITUTION: The method for inducing vascular growth in tissue of a mammal comprises the following steps of: isolating endothelial progenitor cells or bone marrow derived stem cells from the mammal; delivering a cytokine or chemoattractant to a target zone of the tissue; and reintroducing the isolated endothelial progenitor cells or bone marrow derived stems cells to the mammal for homing the endothelial progenitor cells or bone marrow stem cells to the target zone of the tissue for effecting vascular growth at the target zone. The method additionally contains: effecting vascular regeneration by vasculogenesis; effecting vascular regeneration by angiogenesis; effecting vascular growth by arteriogenesis; isolating endothelial progenitor cells from blood of the mammal; isolating bone marrow derived stem cells from the mammal; culturing and proliferating the isolated endothelial progenitor cells and bone marrow derived stem cells in vitro; gene processing of the endothelial progenitor cells or bone marrow derived stem cells to produce therapeutic protein or the like.