METHODS OF EXTRACTION AND PURIFICATION FROM STEVIA REBA UDIANA OF COMPOSITIONS WITH ENHANCED REBAUDIOSIDE-M CONTENT, USES OF SAID COMPOSITION AND NATURAL SWEETENER COMPOSITIONS WITH SAID COMPOSITION

摘要

A method for purifying Reb M includes preparing a crude mother liquor/Stevia rebaudiana primary extract, passing a solution of this primary extract through a multi-column system including a plurality of columns, in series, packed with a porous adsorbent resin to provide at least one column having adsorbed Reb M and eluting fractions with Reb M content from a final/last column in the series to provide an eluted solution with Reb M content.