METHOD FOR PURIFICATION OF DNA FREE-POLYMERASES AND ACCESSARY ENZYMES FOR MOLECULAR DIAGNOSIS USING POLYMERASE CHAIN REACTION

摘要

The present invention relates to a kind of purifying enzymes in polymerase chain reaction, and more specifically, it is related to the DNA for actually removing external contamination, includes enzyme for polymerase chain reaction by sub-fraction (PMA) in processing ethidium monoazide (EMA) or propidium diiodide monoazide. The method according to the invention, the DNA as external contamination are removed efficiently from host cell, and this method can provide highly reliable result by excluding false positive results since the nucleic acid of pollution is when polymerase chain reaction is executed using the enzyme present invention. In addition, this method may greatly strengthen the validity of polymerase chain reaction, and the molecular diagnosis that therefore can improve precision and reliability uses the polymerase chain reaction. ;The 2016 of copyright KIPO submissions