PURIFYING METHOD FOR REMOVING DNA DECONTAMINATION OF POLYMERASE AND COENZYME FOR MOLECULAR DIAGNOSTIC TEST USING POLYMERASE CHAIN REACTION

摘要

The present invention relates to a purifying method of an enzyme used in a polymerase chain reaction and, more specifically, a method for substantially removing exogenous contaminating DNA by treating ethidium monoazide (EMA) or propidium monoazide (PMA) to a fraction including an enzyme used in a polymerase chain reaction. According to the present invention, by effectively removing exogenous contaminating DNA caused from a host cell, it is possible to provide a highly-reliable result by excluding a false positive result due to a contaminated nucleic acid when the polymerase chain reaction is performed by using the enzyme. In addition, it is also possible to highly improve the efficiency of the polymerase chain reaction and increase accuracy and reliability of molecular diagnosis using the polymerase chain reaction.;COPYRIGHT KIPO 2019