Diagnosis by Random Amplified Polymorphic DNA Polymerase Chain Reaction of FourCryptic Species Related to Anopheles (Nyssorhynchus) Albitarsis (Diptera: Culicidae) from Paraguay, Argentina and Brazil

摘要

Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) analysiswas applied to samples from widespread populations of the poorly characterized Anopheles (Nyssorhynchus) albitarsis Lynch-Arribalzaga species complex, and 4 genetically differentiated species were distinguished. A screen of 65 random decamer oligonucleotide primers identified 12 primers, which produced 19 reproducible species-specific genetic markers and 4 markers common to 2 or more species. These markers were correlated in nearly all individuals of each species throughout the ranges sampled, including populations as far apart as 2,500 km. Each individual analyzed was from a different isofemale progency brood, with associated morphological specimens. These specimens will facilitate studies to relate these species to previously reported chromosomal and enzymatic variation as well as to their feeding behavior and potential as malaria vectors. We hypothesize that 3 of the species have recognized valid names: An. (Nys.) albitarsis Lynch-Arribalzaga, An. (Nys.) marajoara Galvao and namasceno, and An. (Nys.) deaneorum Rose-Freitas, whereas the 4th is undescribed.