A set of synthetic oligodeoxyribonucleotides for the detection of the map1α rickettsia gene Anaplasma marginale by real-time polymerase chain reaction

摘要

A set of synthetic oligodeoxyribonucleotides for detecting the map1α rickettsia gene Anaplasma marginale by real-time polymerase chain reaction, containing two oligodeoxyribonucleotide primers and a fluorescently-labeled DNA probe, which have the following structure: Direct primer: F: 5TTGTTTGTGTGTG Reverse primer: R: 5′-CTTCTGCTGATCTTCCTGTCTC-3′Fluorescence-labeled DNA probe: Probe: 5′-VIC-CGCTGATTTGGGGCTGCCTGGCACT-BHQ2