SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES FOR DETECTION OF msp1α RICKETTSIA Anaplasma marginale GENE BY 'REAL TIME' POLYMERASE CHAIN REACTION

摘要

FIELD: biochemistry.;SUBSTANCE: invention relates to biochemistry. Disclosed is a set of synthetic oligodeoxyribonucleotides for detection of msp1α rickettsia Anaplasma marginale gene by polymerase chain reaction in real time. Set contains two oligodeoxyribonucleotide primers and fluorescent-labelled DNA-probe. Forward primer has a structure 5'-TGTTTGGAACCTAGGCTTATAG-3'; reverse primer has a structure 5'-CTTCTGCTGATCTTCCTGTCTC-3'; fluorescent-labelled DNA-probe has a structure 5'-VIC-CGCTGATTTGGGGCTGCCTGGCACT-BHQ2.;EFFECT: higher reliability of detecting Anaplasma marginale, simplification of analysis and shorter duration thereof.;1 cl, 3 dwg, 1 tbl