Method for Separating AND PURIFYING Su00c9RICA bovine Albumin from plasma Membranes using Powder Technology

摘要

Method for Separating AND PURIFYING Bovine Serum Albumin from plasma Powder using Membrane Technology, characterized in that it comprises a First Step where the diluted plasma was added SODIUM CAPRYLATE 0.5 m to obtain a final molarity of ions between 0.011 and 0.0 13 m; a second step of ultrafiltration Volume Reducing and Eliminating Water, Fatty Acids, salts,Peptides and organic molecules and concentrating BSA until a Predetermined value by Continuous removal of permeate volume and maintaining controlled Recirculation Velocity, the permeate Flow.The transmembrane pressure and temperature; a Third step of Elimination of impurities; a Fourth Step ultrafiltration and diafiltration Step Discontinuity and a Fifth of the microfiltration of BSA and Packaging containing solution with different concentrations.